Osmosis

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1
What is osmosis?
The diffusion of water molecules across a partially permeable membrane.
2
What direction does osmosis occur?
From a region of higher water potential to lower water potential.
3
What is water potential?
The likelihood of water molecules to diffuse out of or into a solution.
4
What has the highest water potential?
Pure water.
5
What happens to water potential when solutes are added?
It becomes lower (more negative).
6
What happens if two solutions have the same water potential?
They are isotonic.
7
What factors affect the rate of osmosis?
Water potential gradient, surface area, and thickness of the exchange surface.
8
How does a steep water potential gradient affect osmosis?
It increases the rate.
9
How does the thickness of the exchange surface affect osmosis?
Thinner surfaces speed up osmosis.
10
How does surface area affect osmosis?
Larger surface areas increase the rate.
11
What are serial dilutions?
A step-by-step process to create solutions of decreasing concentration.
12
How do you make a serial dilution?
Dilute a concentrated solution by a factor of 2 multiple times.
13
What is the starting solution concentration for a serial dilution?
2M sucrose solution.
14
How do you dilute for serial dilutions?
Add 1cm³ of the previous solution to 9cm³ of water.
15
What is the point of using serial dilutions?
To test solutions with different water potentials.
16
What is the water potential of a potato cell?
It’s where no mass is gained or lost in the potato.
17
How do you test water potential of potato cells?
Place potato chips in solutions of different concentrations.
18
What happens to potato chips in a lower concentration solution?
They gain water and increase in mass.
19
What happens to potato chips in a higher concentration solution?
They lose water and decrease in mass.
20
How do you calculate the scale factor for a solution?
Divide the concentration you want by the stock solution concentration.
21
What does the calibration curve show?
The concentration at which the potato’s water potential equals the solution’s.

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