photosynthesis

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1
what is photosynthesis?
“how plants make food”
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equation for photosynthesis (in words)
carbon dioxide + water + light yields sugar + oxygen
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equation for photosynthesis
6CO2 + 6H2O + energy —> C6H12O6 + 6O2
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reactants of photosynthesis
carbon dioxide, water, sunlight
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products of photosynthesis
glucose, oxygen
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what organelle is responsible for photosynthesis?
the chloroplast
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where does the plant get water for photosynthesis?
veins (and roots from osmosis)
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where does the plant get carbon dioxide from for photosynthesis?
the stomata. it takes in carbon dioxide and gives of oxygen
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where does the plant get light from for photosynthesis?
from the chloroplasts, located in the mesophyll
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how does the plant move food?
the phloem
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what are the parts of the chloroplast?
the inner and outer membrane, the thylakoids stacked in stacks called grana/ granum, and the liquid inside called the stroma.
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what is the electromagnetic spectrum?
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what are the two stages of photosynthesis?
the light dependent reactions and the calvin cycle
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where do the light dependent reactions occur?
in the thylakoid
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what is needed to start the light dependent reactions?
water and sunlight
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what comes out of the light dependent reactions?
ATP, NADPH, O2
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what happens in the light dependent reactions?
Light hits the pigment cells in photosystem II, which excites them into moving an electron through photosystem II into electron carriers into photosystem I. more light hits photosystem I, which again excites the cells into moving an electron, which turns NADP+ into NADPH.
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photosystem II
the first photosystem (AKA P680). splits the water brought into the cell into H2 and O. the Oxygen is spit out as it is not needed. The hydrogen and its electrons are used in the electron transport chain and ATP synthase.
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electron transport chain
series of electron carrier proteins that shuttle high-energy electrons during ATP-generating reactions
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what is chemiosmosis?
the use of energy in a H+ gradient to drive cellular work
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the two two protein carriers in the electron transport chain
plastoquinone (PQ) and protein complex (PC)
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photosystem I
the second photosystem (AKA P700). creates NADPH from NADP+ using the electrons from hydrogen.
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ATP synthase
Large protein that uses energy from H+ ions to bind ADP and a phosphate group together to produce ATP
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also ATP synthase
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where does the calvin cycle occur?
in the stroma
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what goes into the calvin cycle?
ATP, NADPH, CO2, RuBP
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what comes out of the calvin cycle?
G3P, ADP, NADP+, RuBP
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what happens in the calvin cycle?
three stages: the carbon fixation, reduction, and RuBP regeneration
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carbon fixation:
Three molecules of carbon dioxide are added to three molecules of a five-carbon sugar abbreviated RuBP. These molecules are then rearranged to form six molecules called 3-PGA, which have three carbons each.
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reduction:
PGA is converted to G3P with high energy electrons from NADPH and energy from ATP
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RuBP regeneration:
The remaining G3P is used in reactions that use ATP to regenerate RuBP
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how many times does the calvin cycle have to run to produce one glucose molecule?
twice
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what is G3P?
glyceraldehyde 3-phosphate. A molecule that is made in the Calvin cycle; glucose is formed when two of these molecules combine
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what is RuBP?
ribulose biphosphate; a five-carbon carbohydrate that combines with CO2 to form two molecules of PGA in the first step of the Calvin Cylce.
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what is RUBISCO?
an enzyme that adds CO2 to RuBP in the carbon fixation stage
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all together, this is photosynthesis:
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Leo the lion says Ger
LEO: lose electrons = oxidation GER: gain electrons = reduction
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NADP+ —> NADPH is a…
reduction
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pigments
chlorophyll a + b, anthocyanin, xanthophyll, carotenes/carotenoids
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Chlorophyll A + B
the two types of chlorophyll. A is lighter than B
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anthocyanin
a red pigment
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carotenes/carotenoids
orange pigment
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xanthophyll
yellow/ brown pigment
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when light hits a pigment molecule:
it can absorb (what we see//what is used in photosynthesis), reflect (feel), or transmit (go straight through)
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chromatography equation
rf = $\frac{dye}{solvent}$
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solution = solute + solvent
solute: Substance being dissolved solvent: substance dissolving another substance ex: salt water. salt is the solute, water is the solvent.
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rf values:
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spectrophotometry
measuring how light wavelengths change things. learn about the substance and how it reacts. measures transmittance and absorbance
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what is dpip?
dichlorphenolindophenol - a dye used to monitor light reactions in photosynthesis (NADPH production). competes with NADP+ to grab high energy electrons
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