Chapter 4

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1
You are viewing an image from a microscope in which the specimens in the smear have more than one color. You are probably looking at:
a) a specimen on a dark-field microscope.
b) a scanning tunneling micrograph.
c) either a specimen stained with a differential stain or fluorescent molecules.
d) a smear stained with a differential stain.
e) a specimen stained with various fluorescent molecules.
c) either a specimen stained with a differential stain or fluorescent molecules.
2
In Gram staining, ethanol-acetone is used as a:
a) drying agent
b) primary stain
c) counterstain
d) decolorizing agent.
e) mordant
d) decolorizing agent.
3
Acidic dyes are commonly used for ________ stains.
a) negative
b) acid-fast
c) Gram
d) flagellar
e) endospore
a) negative
4
Specimens are prepared for ________ microscopy using electron-dense stains.
a) scanning tunneling
b) bright-field
c) confocal
d) transmission electron
e) atomic probe
d) transmission electron
5
Images of living specimens can be produced using atomic force microscopes. (TRUE/FALSE)
TRUE
6
The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).
APERATURE
7
Iodine is a (decolorizer/dye/mordant) in the Gram stain procedure.
MORDANT
8
Which of the following is an incorrect pairing?
a) dark field: high contrast
b) magnification; refraction of radiation
c) electron beams; shorter wavelength
d) contrast; staining techniques
e) numerical aperture; curved glass
e) numerical aperture; curved glass
9
Lasers illuminate fluorescent dyes on specimens on ________ microscopes.
a) fluorescent
b) bright-field
c) confocal
d) dark-field
e) phase-contrast
c) confocal
10
The placement of an organism into a domain is made on the basis of:
a) cell ultrastructure
b) G + C content
c) serological tests
d) ribosomal RNA
e) Gram-stain reactions
d) ribosomal RNA
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