1What is the average size of bacteria?
0.2- 0.1 micro m x 2-8 micro m
2Basic Bacteria shapes
Coccus, Rod/Bacillus, Spiral
3Arrangements of Cocci
Pairs- diplococci; Clusters- staphylococcus; Chains- streptococci
4Different Spiral bacteria
Vibrio- 2um; Spirillum- 4um; Spirochete- 1um
5Unusual Bacteria shapes
Star-shaped Stella, Square Haloarcula
6Monomorphic
single shape- most bacteria
8Slime Layer vs S-layer
Slime layer is loose and unorganized; S-layer is composed of protiens
9What is the structure, function, and location of the glyocalyx?
protect cell, traps water, prevents access to potential danger, sticks to surfaces; large branched polysaccarides attached to protien or lipids
10What is the structure of flagella?
protien-based extensions that are attached to a motor
11Flagella movements
Running (straight), Tumbling (switch directions), and swimming
12Different types of bacteria movements
Flagella, Pilus (twitch/protien grappling hook), Surface attachments (gliding)
13How does the flagella moveespond to stimulus?
On bacteria there are recptors for attractants (ex. entering a gradient). Counter clockwise rotation moves cell toward attractant. Clockwise rotation stops forward motion, so cell tumbles and changes direction
14Flagella in Spirochetes
Endoflagell; anchored at one end of a cell; rotation causes cell to move
15Success of gram stain depends on….
length of time of decolorizing step and age of culture
16Results from loss of cell wall
Protoplast
17Acid fast cell wall
complex cell wall with peptidoglycan and mycolic acid; resistant to staining and damage; sugar-polymer extension in cell wall; capsule- waxy coating, lipid-based, creates protien layer
18Mollicutes
Bacteria without a true cell wall
19Acid-fast stains
used to detect organisms that do not readily take up dyes- detects Myobacterium
20ATP-Binding Cassette Transporters (ABC)
Substrate-binding protien wil bind to solvent and the complex will bind the solvent to to the membrane transporter. Then ATP powers the opening of the channel and movement of solvent into the cell
21ABC Transport Iron Example
Bacterium secretes Siderophore that binds to Fe3+. The ABC bings F3 across the membrane. Inside the cell the iron is released
22Efflux channel
Protien channel- allowx molecules to excreted out of cell- differs between gram +/-
23Secretion Systems T3S
Salmonella triggers cells to undergo endocytosis- lives in vesicle inside of cell. Bypasses receptors by moblizing actin cytoskeleton
24How is bacterial dna organized?
in Nucleoid- circular dna; have attachment point to plasma membrane
25Endospores
Resistant to desiccation, heat, chemicals- creates molecular shell to protect until viable; occurs in some gram positive bacilli
26Sporulation
Endospore formation
27Germination
Return to vegetative state
28Two different types of cell wall
Gram positive vs. negative
29Gram-positive
thick layer of peptidoglycan that contains teichoic acids and lipoteichoic acids
30Gram-negative
Thin layer of peptidoglycan that surrounded by an outer membrane. The outer layer of the outer membrane is lipopolysaccharide
31Capsule
distinct and gelatinous. Allows bacteria to adhere to specific surfaces; allows for some organisms to avoid the body’s defense systems and thus cause disease