Micro Ch 4

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1
What is the average size of bacteria?
0.2- 0.1 micro m x 2-8 micro m
2
Basic Bacteria shapes
Coccus, Rod/Bacillus, Spiral
3
Arrangements of Cocci
Pairs- diplococci; Clusters- staphylococcus; Chains- streptococci
4
Different Spiral bacteria
Vibrio- 2um; Spirillum- 4um; Spirochete- 1um
5
Unusual Bacteria shapes
Star-shaped Stella, Square Haloarcula
6
Monomorphic
single shape- most bacteria
7
Pleomorphic
Multishape
8
Slime Layer vs S-layer
Slime layer is loose and unorganized; S-layer is composed of protiens
9
What is the structure, function, and location of the glyocalyx?
protect cell, traps water, prevents access to potential danger, sticks to surfaces; large branched polysaccarides attached to protien or lipids
10
What is the structure of flagella?
protien-based extensions that are attached to a motor
11
Flagella movements
Running (straight), Tumbling (switch directions), and swimming
12
Different types of bacteria movements
Flagella, Pilus (twitch/protien grappling hook), Surface attachments (gliding)
13
How does the flagella moveespond to stimulus?
On bacteria there are recptors for attractants (ex. entering a gradient). Counter clockwise rotation moves cell toward attractant. Clockwise rotation stops forward motion, so cell tumbles and changes direction
14
Flagella in Spirochetes
Endoflagell; anchored at one end of a cell; rotation causes cell to move
15
Success of gram stain depends on….
length of time of decolorizing step and age of culture
16
Results from loss of cell wall
Protoplast
17
Acid fast cell wall
complex cell wall with peptidoglycan and mycolic acid; resistant to staining and damage; sugar-polymer extension in cell wall; capsule- waxy coating, lipid-based, creates protien layer
18
Mollicutes
Bacteria without a true cell wall
19
Acid-fast stains
used to detect organisms that do not readily take up dyes- detects Myobacterium
20
ATP-Binding Cassette Transporters (ABC)
Substrate-binding protien wil bind to solvent and the complex will bind the solvent to to the membrane transporter. Then ATP powers the opening of the channel and movement of solvent into the cell
21
ABC Transport Iron Example
Bacterium secretes Siderophore that binds to Fe3+. The ABC bings F3 across the membrane. Inside the cell the iron is released
22
Efflux channel
Protien channel- allowx molecules to excreted out of cell- differs between gram +/-
23
Secretion Systems T3S
Salmonella triggers cells to undergo endocytosis- lives in vesicle inside of cell. Bypasses receptors by moblizing actin cytoskeleton
24
How is bacterial dna organized?
in Nucleoid- circular dna; have attachment point to plasma membrane
25
Endospores
Resistant to desiccation, heat, chemicals- creates molecular shell to protect until viable; occurs in some gram positive bacilli
26
Sporulation
Endospore formation
27
Germination
Return to vegetative state
28
Two different types of cell wall
Gram positive vs. negative
29
Gram-positive
thick layer of peptidoglycan that contains teichoic acids and lipoteichoic acids
30
Gram-negative
Thin layer of peptidoglycan that surrounded by an outer membrane. The outer layer of the outer membrane is lipopolysaccharide
31
Capsule
distinct and gelatinous. Allows bacteria to adhere to specific surfaces; allows for some organisms to avoid the body’s defense systems and thus cause disease
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